Animal Health Research Unit, St.Xavier
’s College (Autonomous),
Palayamkottai-627856, South India
Issued: May 01, 2010
A phytochemical screening of alkaloids, triterpenes saponins and tannin was carried out the selected medicinal
plant parts of leaf, barks, rhizome and fruit samples from 63 plant species representing 26 genera and 25 families.
The positive results of phytochemical diversity of bioactive constituents were alkaloids (58.73%),
terpenoids (92.06%), flavonoids (90.48%), saponins (50.79) and tannins (31.74%) present in total of sixty three
Tirunelveli Hills; alkaloids; flavonoids.
Natural products once served humankind as the source of all drugs, and higher plants provided most of
these therapeutic agents. Today, natural products (and their derivatives and analogs) still represent over 50% of
all drugs in clinical use, with higher plant-derived natural products representing ca. 25% of the total . The
World Health Organization estimates that 80% of the people in developing countries of the world rely on
traditional medicine for their primary health care, and about 85% of traditional medicine involves the use of
plant extracts. This means that about 3.5 to 4 billion people in the world rely on plants as sources of drugs .
More than 80 % of the world
’s populations depend on traditional plant-derived medicines for their health needs.
The use of plants for medicinal purposes represents the largest use of biodiversity in the world. Many more species
of plants are used as medicines, for example, than are used for food. Focusing on medicinal plants, therefore, has
the potential for involving people more widely in conservation issues.
Conservative estimates suggest that there are more than 250,000 species of higher plants existing on this planet,
and only a very small percentage of plants have been exhaustively studied for their potential value as a source of
drugs. Obviously natural products will continue to be extremely important as sources of medicinal agents.
Discovery of new drugs from plants requires the screening of many thousands of plant extracts, and thus
requires continued access to the vast plant biodiversity of the Earth, much of which is located in tropical rain
forests. Tropical forests cover only 7% of Earth surface, but they are thought to contain at least one half of all
plant species. In these forests, deforestation is proceeding at a rate of 20 million ha/year, resulting in the loss of
species at rates estimated to be 100 to 1000 times greater than background extinction. Therefore, the main
objectives of this study to identify in the phytochemicals constituents of wild plants collection from the
Tirunelveli hills of Southern Western Ghat region, Tirunelveli District, South India.
Plant samples were collected from Tirunelveli hills, Southern Western Ghats region, Tirunelveli District, South
India (Fig.1) and during the periods from 1997-2007. 50gms of dried powdered materials (Plant name and plants
parts listed in table -1) were separately extracted with 50% ethanol at room temperature. Extracts of each plant
were stored in the refrigerator at 4°C prior to use of phytochemical analysis. The presence of all alkaloids
was determined the Culvenor and Fitzgerald methods . For steroids or triterpenes, the Liebermann-Burchard
test was used , the froth test for saponins  and extract react with lead acetate solution to form white
precipitate test for tannin .
Results and Discussion
The result of phytochemical screening of sixty-two plants giving positive or negative tested for alkaloids,
terpenoids, flovanoids, saponin and tannin are listed in Table -1.
Alkaloids: Sixty- three plants represent 25 families and 26 genera; 58.73% of the plants tested positively for
alkaloids. Presence of active chemicals of alkaloids in the aqueous phase was detected by the formation of a
precipitate on addition of Mayer
’s reagent (K
). Alkaloid isolated from A. scholaris was reported
previous workers [7-8]. Hadi and Bremner,  reported 49 families and 80 genera, and this is an indication of
alkaloid reported in the variety of Lombok medicinal plants. Of these plants, twenty-three species (23%)
contained alkaloids. In a survey of plants of Tasmania, Australia, which focused mainly on endemic species in
this cool temperate environment , 15% of the plant species gave positive alkaloid tests. However, in a
similar alkaloid survey in Queensland, Australia, with many tropical and sub-tropical species, 20% of species
were positive .
Negative results were obtained such as Bauhinia purpurea, Bauhinia malabarica, Alstonia macrophylla,
aluminum chloride solution in methanol concentrated HCl, magnesium turnins, and potassium hydroxide
solution. Negative results were obtained such as Curculigo orchioides, Dioscorea pentaphylla, Dioscorea
alata, Memecylon umbellatum, Pavetta indica and Psychotria beddomei.
Saponin: A total of 32 species (50.79%) gave positive reactions for saponins. Very strong reactions were observed
in fruit samples belonging to Dioscorea pentaphylla, Dioscorea alata, Diospyros malabarica, Diospyros
Table 1: Survey of Phytochemical diversity of secondary metabolism of wild plants.
Earlier studies on phytochemical screened for their chemical contents such as alkaloids, triterpenoids and
saponins found to be 205 plant species . Ahmad and Mat-Salleh (1988) had reported some screening on 148
plant samples. Phytochemical screenings were also carried out on plants collected from Tawau Hills Park ,
and SayapKinabalu Park . The last phytochemical screening reported was for alkaloid contents of plants
from Gunung Danum area .
The present study was carried out the phytochemical analysis of 63 medicinal
In conclusion, results of phytochemicals were strong color indicated for active compounds present in Tirunelveli
hills of Western Ghats region, South India. Moreover, there is a need to conduct isolation and
identification phytochemicals from plant species of Western Ghats.
The author would like to acknowledge SERC-DST,New Delhi, for financial support.
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